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Image Search Results
Journal: Mrs Communications
Article Title: Simple dynamic cell culture system reduces recording noise in microelectrode array recordings
doi: 10.1557/s43579-024-00554-3
Figure Lengend Snippet: Electrophysiology recordings in static and dynamic cell culture system. (a) Representative signal traces are shown for electrophysiology recordings in static (light green first row) and dynamic (light violet second row) cell culture systems without cells as well as dynamic (violet third row) cell culture system with cells when injecting 80 µM 4-Aminopyridine at 0 s. Signals were filtered (50 Hz notch filter, 100 Hz low-pass filter, downsampled from 20 kHz to 200 Hz) and detrended for further analysis. (b) Electrical noise is calculated as root mean square of recorded voltage spikes exceeding ± 50 μV and summarised (mean ± standard deviation) for all functioning circular electrodes (opening diameter 100 μm, static cell culture system: n = 44, dynamic cell culture system: without cells n = 16, with cells n = 28). While no significant difference can be observed when comparing the dynamic culture without and with cells ( p adj = 6.76 × 10 –2 ), the static system shows significantly higher noise (Kruskal–Wallis test, post hoc Dunn’s test) compared to the dynamic system without cells ( p adj = 2.64 × 10 –12 ) or with cells cultured for 2 days prior to experimentation ( p adj = 7.57 × 10 –10 ).
Article Snippet: Recordings were performed with an
Techniques: Cell Culture, Standard Deviation